Abstract:

Somatostatin could inhibit the growth of the tumor cell, and the correlation between somatostatin and the tumor is becoming a hot topic in oncology. In our research, MFC cells were infected with the lv-shRNA viral stocks targeting somatostatin with pcDNA3.1-SS transfected cells as the positive controls. The results showed that high infection efficiency was gained at 96 h with the fluorescence observation. SS protein level in LV-shRNA infected cells were reduced 49.55% in protein level (P<0.05) detected with RIA. Cell growth curve measured using the MTT assay showed that high levels of SS inhibit the cell growth ability significantly (8.14%, P<0.05). Reversely, lowering SS could stimulate the cell growth ability (19.27%, P<0.05), which identified that SS could inhibit the growth of the tumor cells MFC. This is the first research of the inhibition effects of SS on cell growth via high and ow levels of SS in vitro, and lays theoretical basis for the SS or the SS analogue on the oncotherapy.