Abstract:

The experiment provided the method basis for the Glycyrrhiza uralensis callus’ subculture and further studies. Taking callus from different sources of Glycyrrhiza uralensis as trial material, the cell size of callus in different culture stages were observed with paraffin section, and the changes of mitochondria shape and the size from Glycyrrhiza uralensis callus in the different culture stages, was observed with pendent drop. The results showed that the length of cell from callus had a significant effect on source of explant (P<0.5), the length of cell from leaf callus (average 29.41 μm) was greater than from rhizome’s (average 25.67 μm), but was smaller than from bud’s (average 31.40 μm). The length of cell from callus had a very significant effect on their development stages(P<0.01), the largest cell (average 37.45 μm) appeared after 30 days’ culture, and then reduce ,when cultured 40 days, the average length was reduced to 29.55μm.The number of dumbbelllike mitochondria in the cell from bud and leaf callus was more than rhizome’s. It had a notable interaction in size of mitochondria, the source of explant and the period of culture (P<0.05). The length of mitochondria from rhizome callus was 2.60 μm (maximum) after 30 days’ culturing, while bud’ 2.07 μm and leaf’ 2.01 μm after 40 days’ culturing. The culture stage had a very significant effect on the size of mitochondria (P<0.01), when 30 days’ cultivation, the average length of cell can reach to 2.06 μm (maximum). This finding laid the foundation for Glycyrrhiza uralensis callus successive subculture, simultaneously to provide the cytology aspect basis for differentiation and the redifferentiation for the Glycyrrhiza uralensis.