Abstract:

In order to study the toxicity and possible lethal mechanism of supercritical extract of Stellera chamaejasme L., the experiment was conducted. In this study, the contact toxicity of supercritical extract of Stellera chamaejasme L. (SCE) to Tetranychus cinnabarinus was tested using slide dip method, and dynamic toxicosis symptoms of SCE-exposed mites were also detailedly observed. The results showed that the contact toxicity LC50 value of SCE to T. cinnabarinus was 2.411 mg/mL at 24th h post-treatment. Some typical neurotoxic symptoms such as excitement and convulsions were observed in SCE-exposed mites. Subsequently, the changes of activities of AChE, MAO, Na+-K+-ATPase and Ca2+-Mg2+-ATPase after exposed to SCE were assayed. The experiment showed that SCE could significantly inhibit the activities of AChE, MAO, Na+-K+-ATPase and Ca2+-Mg2+-ATPase. The results led us to believe that SCE may be a neurotoxin.