Abstract:

The genetic diversity of prolamine among 76 cultivated buckwheat accessions (55 accessions of tartary buckwheat, 21 accessions of common buckwheat) from 7 countries were analyzed by A-PAGE. A total of 18 prolamine bands were separated by electrophoresis. The abundant variations of prolamine were observed, and 18 prolamine bands were detected, ranging from 6 to 12 bands per accession with the average of 9.5, among which 88.89% bands were polymorphic. The genetic similarity (GS) varied from 0.389 to 1.000, with the average of 0.777. The cluster analysis showed that all the accessions could be clustered into two groups at GS=0.63 level, tartary buckwheat and common buckwheat. Moreover, the accessions from the same origin frequently could be divided into one group, which indicated that there was a significantly positive correlation between genetic differentiation and geographical habits among the cultivated buckwheat.