Abstract:

The stem segment of Pellionia repons (Lour.) Merr. was used as explants to study the effect of surface sterilization methods and hormone combinations on callus induction, adventitious shoots and plantlet rooting. The best sterilization method was that the explants were surface sterilized with 70% alcohol for 15 s and rinsed one time with sterile water, disinfected with 0.1% mercuric chloride for 6 min and rinsed one time, and then soaked in 0.1% mercuric chloride for 6 min and rinsed four times. Optimal media appeared to be: MS+3.5 mg/L 6-BA+0.1 mg/L NAA for callus induction, MS+2.0 mg/L 6-BA+0.1 mg/L NAA for shoot induction and multiplication, and MS+0.3 mg/L NAA for rooting. The survival rate of plants after transplanted to glasshouse was 91% and the plants grew well.