Abstract:

The effects of centrifugal force and centrifugation time on callus induction and growth of anthers of Medicago truncatula Gaertn F1 hybrids from different genotypes were detected in this study. The results showed that centrifugation could promote the formation of anther callus, especially for the genotype A anthers. The callus induction rates of anthers were significantly different (P<0.01) after given different centrifugal force, the optimum pretreatment was 1000×g for 2min. Callus growth was not obviously affected by centrifugal force and centrifugation time. However, the strength of peroxidase (POD), catalase (CAT) and superoxide dismutase (SOD) of callus were affected by centrifugal force. POD activity gradually increased with the increase of centrifugal force, and the trend of it was opposite with SOD and CAT.