Abstract:

In this experiment, Medicago truncatula plants were used to study the correlation between microspore development and bud morphology, de-differentiation capacity of the anther was also determined. The results showed that microspore development of Medicago truncatula could be divided into three periods: early-or mid uninucleate, late-uninucleate and binucleate. A significant correlation could be found between development stages of microspore and buds morphology. Anthers at different developmental stages judged by buds morphology were cultured to detect the capacity of dedifferentiation. The anthers with late-uninucleate microspores had the highest callus induction rate of 37.21%, followed by 4.82% of early-or mid uninucleate microspores, but the anthers with binucleate microspores could not dedifferentiate. This data suggested that anthers with late-uninucleate microspores of Medicago truncatula are the optimal materials for dedifferentiating, and at this period, the buds swells with green sepals shorter than petals.