Abstract:

Protein samples, prepared with water extraction or acetone precipitation, were both well separated by two-dimensional electrophoresis (2-DE) after purification with liquid-phase isoelectric focusing (LIEF). So LIEF was an effective purification method for edible birds’ nest (EBNs). With different preparation methods, similar 2-D gel protein profiles were obtained in two white EBNs and one blood EBN. While compared to dialyzation and lyophilization, the time of sample preparation with acetone precipitation was greatly reduced. Consequently, protein precipitated with acetone prior to LIEF was more applicable to blood EBNs for their low protein extraction rate.