Abstract:

In the present study, to find out the better methods of soybean powder genomic DNA extraction for the molecular marker analysis of high quality and sufficient samples, the extraction method of genomic DNA from soybean powder purchased from market was optimized. There are 12 methods used in the extraction of DNA, in which pyrolysis, high-salt low-pH, isopropyl alcohol, CTAB, SDS or GuSCN, and their modified methods were involved. The effects of different methods were compared by detecting the DNA by optical density, agarosegel electrophoresis and polymerase chain reaction (PCR). Results showed that the genomic DNA extracted by all methods except for modified High-salt low-pH method could be used in PCR reaction. Meanwhile, the better DNA concentration and purity will be gained by different methods in the order of modified pyrolysis method, modified isopropyl alcohol method, pyrolysis method, isopropyl alcohol method, High-salt low-pH method, modified CTAB method, CTAB method and modified SDS method. These methods are simply to operate, fast to gain results, and suitable for the extraction of total DNA from soybean powder.