Abstract:

Phenols, polysaccharide protein and other secondary metabolism substances existing in tissues or organs which influence total RNA extracting from Chrysanthemum. In this study, the total RNA from leaves. roots. shoots and flowers of Chrysanthemum were extracted by improved Trizol method and was detected by gel electrophorese and RT-PCR. The results showed that the total RNA were high purity and the strips of it was clear and complete. The A260/A280 detected by UV-Spectroscopic was 2.08-2.19. Partial gene of Actin was achieved by RT-PCR from leaves. The improved Trizol method was quick and efficiency for extracting total RNA from Chrysanthemum. It was a common method to isolate total RNA from different tissues and organs of Chrysanthemum.