Abstract:

Stems of hybrid clone of Populus deltoids and Populus ussuriensis were used as explants to study its in vitro culture and leaf regeneration system, and to explore effect of different prescriptions of hormone on induction, differentiation, propagation of adventitious bud, and rooting. The results showed that, the optimal sterilization time was 5 min, the optimum medium for multiplication was MS + 6-BA 2 mg/L +NAA 0.1 mg/L + ZT 0.5 mg/L, medium with MS + 6-BA 0.5 mg/L + NAA 0.05 mg/L might achieve the proliferation and restoration effect on adventitious bud, and the optimal medium for rooting was MS + NAA 0.3 mg/L. Among the five hybrid clones, No.177 showed the best differentiation potential, its regeneration ability for leaf adventitious shoot was significantly higher when cultured with the abaxial side upward than with the adaxial side downward, its corresponding differentiation ability with leaf, stem and root segment come out to be in a descending order. This study had optimized the tissue cultural system of hybrid clone of P. deltoids and P. ussuriensis, result of which had laid groundwork foundation for its rapid propagation and genetic transformation analysis.