Abstract:

In order to reveal the structure and function characteristics of TaSnRK2.9 in wheat, and provide the basis for the research and application of this gene, the cDNA of TaSnRK2.9 was cloned in wheat by RT-PCR based on the nucleotide sequence of SAPK9 gene in rice, and the bioinformatics analysis was also finished. The results showed that the length of ORF was 1092 bp, encoding 363 amino acids, Sequence analysis and the phylogenetic trees showed that this gene was closely related to SAPK9 (AB125310) of rice, belonged to the subfamily Ⅲ. Subcellular localization predicted TaSnRK2.9 gene located in the cytoplasm. Gene expression profile analysis found that TaSnRK2.9 gene expressed in roots, stems, leaf, flowers, and was more highly expressed in leaf. The cloning of the TaSnRK2.9 gene full length cDNA provides the basis and guidance for its characteristics and function.