Abstract:

The experiment was purposely to research the effect of phospholipase D on the freezing tolerance of Arabidopsis thaliana, reveal the mechanism of plants freezing tolerance and phospholipid signal transduction. Arabidopsis thaliana mutant PLDγ1 or PLDγ3 was deficient and wild-type was used as materials. The two mutants and wild-type were cold acclimated and freezing stressed using a climate chamber. There were no significant differences between the ion leakage of the mutants and wild-type whether cold acclimation freeze or non-acclimation freeze. The results showed that PLDγ1 and PLDγ3 neither mediate regulation of constitutive freezing tolerance nor low temperature signaling.