Abstract:

[Objective] to supply sequence information of internal control gene for analyzing gene expression of I. batatas and viruses infecting I. batatas. [Method] Sequences of 18S rRNA gene were cloned using PCR method from genomic DNA of I. batatas cultivar ‘Shangshu19’, ‘Beijing553’ and I. setosa, respectively. [Result] The sequencing of the DNA fragments all generated a total of 1630 bp nucleotide sequence. The obtained 18S rRNA gene sequences of I. batatas and I. setosa shared more than 98% identity with I. hederacea and Nicotiana tabacum among dicotyledons, and shared high identity with Lilium superbum among monocotyledons. [Conclusion] Partial sequences of 18S rRNA gene were cloned from genomic DNA of I. batatas cultivar ‘Shangshu19’, ‘Beijing553’ and I. setosa, which provided sequence information not only for analyzing gene expression of I. batatas and viruses infecting I. batatas using 18S rRNA gene as internal control, but for molecular systematic research of I. batatas and I. setosa.