Abstract:

To analyze cassava resource with different phosphorus contents in root starch by using the RAPD genetic background research method from the molecular level, which would lay foundation for the cassava breeding with high phosphorus in root starch. We chose 30 selected cassava varieties with different phosphorus in root starch as the material, which were from the cassava germplasm nursery in the Tropical Crops Genetic Resources Institute of Chinese Academy of Tropical Agriculture. The improved CTAB method was used to extract DNA, and then was for PCR amplification through 18 RAPD primers. The amplification products were conducting the genetic relationship analysis by NTSYS software. The results showed that DNA extracted by the improved CTAB method could be used to do RAPD analysis. Using 18 RAPD primers totally obtained 131 amplification bands, of which were 126 polymorphic bands and the percentage of polymorphism was 96.2%. It didn’t find specific band related with phosphorus content. The cassava resources with similar phosphorus content in root starch can not significantly cluster. The genetic similarity coefficient (GS) between 30 resources was more than 0.71. The RAPD results demonstrated that there was no obvious genetic relationship between different phosphorus contents in cassava root starch and also found no specific genes associated with difference in phosphorus.