Abstract:

【OBJECTIVE】Investigate effects of Aphidius gifuensis teratocytes on Physiological Metabolism of aphis and change themself. 【METHOD】In the study, folin-phenol,SDS-PAGE,thin layer chromatography and Soxhlet extractor method was used to determine the content of protein,protein composition,the quantity of sugar and glyceride for haemolymphe in parasitized and non-parasitized host【RESULTS】It is indicated that the metabolism of parasitized aphid was constantly inhibited as compared with that of the non-parasitized host. The level of protein and glyceride is increasing with instars to maximum in the third day, then lower, but suger content declined gradually, may be due to secretion and synthesis of teratocytes .At the same time, SDS-PAGE analysis showed that 15.9kDa and 51.1kDa parasitic specific protein was appeared in the hemolymph. 【CONCLUSION】Through the analysis of main components for haemolymphe in parasitized and non-parasitized host, Aphidius gifuensis teratocytes plays an important regulatory role to the development of the host.