Abstract:

In this study, resistance gene homology cDNA sequence from wheat was isolated by using homology-based method. The primers were designed according to the conserved sequence of resistance gene and a 525 bp fragment was isolated from TcLr24. BLASTp analysis showed that the deduced amino acids of protein consisted of a NBS conserved domain, which were identical to the conserved domains of many plant resistance genes. The sequence appeared to be induced by Puccinia triticina and was induced genes in the wheat leaf tissue by semi-quantitative RT-PCR. The sequence which provide the short cut for researching of wheat resistance gene.