Abstract:

In order to obtain Newcastle disease virus genome-wide cDNA of La Sota strain through a series of primers splicing. According to SOE PCR method, it was required to design primer. As for NDV La Sota strains, first sectional amplification was carried out, and then applied the SOE PCR method to connect the fragments. Genome-wide connections could obtained about 15000 bp amplified fragments. It’s turned out that the resulting product was genome-wide cDNA of the NDV La Sota. The results showed that the gene sequences which synthesised by SOE PCR method was sequenced and digest by restriction enzyme. It’s turned out that the gene sequences were consistent with the expected results. This study laid the foundation for the construction of Newcastle disease La Sota strain’s infectious cDNA. It also provided a reliable guarantee for the further study of the biological properties of NDV, the relations between its structures and functions, and to explore the factors that affect NDV virulence, as well as the development of new vaccines.