Abstract:

Quality control technology of malachite green residues in aquatic products were carried out to filter out the appropriate storage and transportation conditions for drug verification, with an aim to minimize the impacts of external factors on the tests results and to ensure the fairness of the outcome. Cod and carp were used as substrates to analyze the degradation of malachite green over time in 3 different concentrations(1.5, 5, 50 μg/kg）and 3 different storage conditions (normal room temperature 25℃, cold storage4℃ and freezing-18℃). The results showed that under different storage conditions, the degradation of malachite green with different substrates in varying concentrations varied and the degradation rate also varied. When cod was taken as the substrate, the degradation under three concentrations showed similar results: degradation under high concentration>degradation under medium concentration>degradation under low concentration. When common carp was taken as the substrate in normal room temperature, it was found that: degradation under high concentration>degradation under medium concentration>degradation under low concentration. However, under cold storage and freezing conditions, the result was different with degradation under medium concentration>degradation under high concentration>degradation under low concentration. It was concluded that different storage conditions had different effects on the malachite green standard addition samples. By the impact of enzyme, short-term preservation of malachite green standard addition samples could be kept at normal room temperature, which was also easy to conduct detection soon. If the samples couldn’t be arranged to be detected within 48 hours, the samples should be preserved by freezing methods, which was a good preservation of its original properties and also a good way to avoid impacts of this test results.