Abstract:

In order to produce microbial protein, taking ‘Bingtang’ navel orange peels as raw material, statistical methods were applied to optimize the culture medium. The optimum auxiliary material, nitrogen source and inorganic salts were obtained by mono factorial experiments, which were bran, ammonium sulfate, ZnSO4 and CaCl2. The central points of response surface methodology were got by steepest ascent experiment, which were auxiliary material 15%, nitrogen source 3% and inorganic salts 0.25%. At last, the experiment results of box-behnken showed that the optimum culture medium was composed of auxiliary material 15%, nitrogen source 3.3% and inorganic salts 0.27%. Under the optimized conditions, true protein content reached 19.97%, which was closed to the maximum predicted value.