Abstract:

To utilize fluorescin gene GFP to detect the express and localization of exogenous gene, plant expression vector pCAMBIAl302-zeolin with a green fluorescent protein gene would be constructed. The total length sequence of zeolin gene in pDHA plasmid was amplified by PCR. The fragment was cloned into pMD18-T middle vector, and a new recombined vector named pMD18-T-zeolin was obtained. A new plant expression vector named pCB-GFP-zeolin was constructed after cutting two vector pMD18-zeolin and pCAMBIA1302 with restriction enzymes, subsequently reclamation, ligation, transformation and identification. Then the recombined plant expression vector pCAMBIAl302-zeolin with a green fluorescent protein gene was transformed into epidemic cells of onion by gene gun method and was detected by confocal microscopy. The recombined plant expression vector pCAMBIAl302-zeolin with a green fluorescent protein gene has been constructed and the fusion gene was transient expressed. It was suggested that the recombined plant expression vector pCAMBIAl302-zeolin with a green fluorescent protein gene was successful. Construction of the plant expression vector might play an important role in genetic transformation, the gene functional identification and breeding of new varieties.