Abstract:

In order to study solid fermentation conditions in Coniothyrium minitans and improve its biocontrol effect, the effect of six natural substrates on solid-state fermentation in C. minitans was compared. Spore of C. minitans was tested at different fermenting media, temperature, initial moisture and inoculation rate. The results showed spore was most in wheat grain and corn grain media and better in wheat bran and cotton seed hulls media. Being cheaper, wheat bran and cotton seed hulls acted as fermentation materials of following test. When substrates contained wheat bran, cotton seed and inorganic salt, the temperature was 20℃, initial moisture of fermentation substrates was 60%, inoculation rate was 7%, spore could reach 1×109 conidia/g at last. Fermenting conditions were further optimized. It showed that rate of wheat grain and corn grain, light, contents of mannose and glycine obviously influenced spore.