Abstract:

This study intended to identify suitable ultrasonic conditions of ChIP which would provide appropriate fragments for the precipitation of antibody in Arabidopsis seedlings (Columbia ecotype). Arabidopsis thaliana tissues were fixed with 1% concentrations formaldehyde for protein to DNA cross-linking, sonicated to shear the DNA into fragments of appropriate size. The results showed that the optimal sonication conditions for ChIP of Arabidopsis thaliana seedlings turned out to be 8 rounds of 20 seconds each time at 65% power, interval of 4 min each time which was sufficient to obtain 400-800 bp size DNA fragments for the subsequent experiment. Suitable ultrasonic conditions of ChIP were identified through the present study. It laid the foundation for the subsequent experiments.