Abstract:

It is aimed to study and help for selecting the germplasm of the resistance gene of the tobacco ralstonia solanacearum in RRS1 gene. The investigation and selection was carried out in 2008, 2009 and 2011. Eighty tobacco germplasm resources were chosen in the world. The investigation to the index of ralstonia solanacearum in tobacco was carried out in the field. The nine pairs of specific primers were selected to screen for the RRS1 gene. Through the selection, the primer 6F-6R (5′-ATGAGAAAGAGGCTCGTCAA-3′ and 5′-ACCACAACCCTCAAGCAGTT-3′) was sure to pick out the available germplasm. Three germplasm of tobacco was selected for containing the specific amplified sequence of RRS1 for G28, K346 and LMAFC34. The specific sequences amplified by 6R-6F, were sequenced in 3 tobacco germplasm resources, and the homology of RRS1 cDNA was compared, these works established the base for assessing the homology of RRS1 gene.