Abstract:

Antagonistic strain E-1of rhizobacteria against the fungi and bacterial pathogen were selected on NA and potato dextrose agar with many rhizobacteria isolated from the rhizosphere soil of Ageratina adenophora Sprengel from Kunming suburbs in Yunnan, respectively. Identification on basis of phenotypic and physiological and biochemical properties from biolog data indicating that strain E-1 homologized with which descripted for genus Bacillus. 16s rDNA determination and analysis was used for further identification, which showed that 16s rDNA sequence of strain E-1 shared 100% homologies with published sequence of Bacillus subtilis GZB from Genbank, and belonged to the same branch in phylogenetic tree. In vitro antagonistic assay showed strain E-1 could suppress the growth of five plant bacterial and five plant fungal pathogens. The results showed that strain E-1 had different level antagonistic activity to the fungi and bacterial pathogen. And maybe potential used for biological control of plant diseases.