Abstract:

Abstract: In order to study the role of CHI and CHS in flavonoids synthesis, a 642bp CHI promoter partial sequence and a 613bp CHS promoter partial sequence were cloned by genome walking PCR. The sequences were analyzed by PlantCARE software. The results showed that the core promoter elements TATA-Box and CAAT-Box were found in both sequences. The cis-acting elements including Box I, ABRE, G-Box were also found in both sequences. The functions of these elements were involved in the abscisic acid responsiveness and light responsiveness. It was indicated that the expression of CHI and CHS was controlled by abscisic and light. The results also found that the expression of CHI and CHS was related to plant hormones and environment.