Abstract:

The internal transcribed spacer (ITS) region of the ribosomal DNA from 11 isolates of Rhizoctonia solani Kühn from turfgrass and 4 from soil were sequenced in order to identify anastomosis groups and discuss phylogenetic relationships between them. Based on the 15 sequences and 30 published sequences of R. solani from 14 anastomosis groups or sub-groups, R. cerealis, R. zeae and R. oryzae in GenBank database, the ITS phylogenetic tree were generated with MEGA 4.1. The result showed that, all the representative isolates of known anastomosis groups and 15 samples tested were clustered into one group. In this group, representatives of the different anastomosis groups clustered separately, and 15 isolates sequenced were divided into AG2-2 and AG1-IA. These results provided a reliable basis for disease control, and also suggested that the sequence analysis of ITS rDNA region was a valuable tool for identifying anastomosis groups of R. solani.