Abstract:

One pair of primer was synthesized according to the sequence of hBD3 published in Genebank. The 1391bp of human β-defensin-3 genomics sequence was obtained including the signal sequence, two exton ,one intron and restriction enzyme site and end code. Compared with the sequence published in NCBI, 99.93% nucleic acids were the same except for A deletion in intron. 100% amino acid were same. The sequence was first inserted into pMD18-T vector, then inserted into the pcDNA3.1 vector which resulted in the eukaryotic expression vector pCDNA-hBD3. This was the base for further study of its expression and function.