Abstract: In order to obtain anthocyanidin synthase (ANS) gene from Lilium. degenerate primers were designed based on sequences blast of ANS genes from other species, and the fragment of ANS gene in Lilium was cloned by homology cloning method. The gene fragment was 701 bp encoding 233 amino acid proteins. Sequence alignment revealed that, the deduced amino acid sequence was 86%, 81% and 77% identical to Tulipa gesnerian, Iris hollandica and Prunus avium, respectively. The ANS expressed at different levels with the highest in petal, the second in leaf and stem. However, there’s no expression in bulb. ANS fragment was successfully cloned from Lilium through this research. This lays a solid foundation for cloning the full length cDNA sequence.there’s no expression in bulb.ANS fragment was successfully cloned from Lilium through tis research.This lays a solid foundation for cloning the full length cDNA sequence.