Abstract: In order to provide theoretical basis for large area cultivation of Matteuccia, the different organic manure treatment experiment was conducted in the artificial control of water ( the most suitable Matteuccia seedling growth) conditions, study on Matteuccia seedlings to different organic manure, different gradient fertilizer treatment reaction mechanisms and the changes of photosynthetic characteristics of Matteuccia.The results showed that the growth of Matteuccia seedlings were affected by the three different sources of organic manure,the effect of manure sort order is Composting of pig manure(Ta)> Composting of cow manure(Tb)> Composting of chicken manure(Tc),but there had no significant trend between the different manure amount.The biomass accumulation of Matteuccia aboveground part was affected by the three different sources of organic manure,the effect of manure sort order is Ta> Tb> Tc>CK, and there had significant trend between the different manure amount.For different applying organic manure,when the application of organic manure was 2000 kg?hm-2, the biomass of Matteuccia aboveground part layer was the highest. Compared with the CK， the aboveground dry weight were increased by 140.27%, 84.16%, and 45.80%,meantime the results showed that extremely significant correlation between Photosynthetic characteristics and biomass accumulation (Especially in Matteuccia above-ground dry weight).