Abstract: To analysis growth restrictive elements of Flaveria bidentis, three habitats(Ⅰwaterside, Ⅱcropland, Ⅲwasteland)were selected to study the stoichiometric characteristics and relationship of plant leaf and invaded soil. In three habitats, Invaded soil C, N, C:P, N:P were Ⅲ>Ⅰ>Ⅱ, PⅠ>Ⅱ>Ⅲ, C:N Ⅲ>Ⅱ>Ⅰ(P<0.05); The leaf C concentration had no significant difference, Ⅰsignificantly higher than Ⅱ and Ⅲ in N or P, but Ⅱ and Ⅲ were significantly higher than Ⅰin C:N, C:P, N:P (P<0.05). The soil P or C:N was significantly positive correlation with the leaf, no significant correlation with the C, C:P, N:P. The soil C was significantly positive correlation with N, no significant correlation with the C-P, N-P. The leaf N was significantly positive correlation with P, while C-N and C-P were no significant correlation. The change was synchronous between soil organic matter and N. N was growth restrictive element of F. bidentis growing in the waterside, while in the cropland or wasteland was P.