Abstract: Explore the influence on the morphological trait variation of Campanula atifolia induced by high temperature. Campanula atifolia, as material, using orthogonal design with temperature, the length of flower bud and processing time as factors were induced by high temperature. The effects of each factor were evaluated by range analysis. The results indicated that the temperature and processing time were the main factors which affect the morphological trait variation of Campanula atifolia. Compared with the original species with purple five petals, new variety plants were obtained, 5 had six petals, 1 had oval petals and 3 had white flower color. The experiment illustrated the effectiveness that induced by high temperature in the development of genetic resources, and would greatly promote the development of breeding technology on petals variation.