Abstract: In order to study the effects of different organic fertilizer and chemical fertilizer on yield and quality of flue-cured tobacco, Field experiment combined with laboratory analysis was employed in this study,We carry out the tests in the same organic nitrogen amount conditions (accounts for 20% of the total nitrogen). The results show that: (1) organic fertilizer can improve the proportion of superior tobacco leaves, improve tobacco yield and value (except soil manure), the yield was increased49.7~219kg/hm2, the value was increased 890.2~3081.1元/hm2. (2) organic fertilizer can make its chemical composition tend to be more harmonious, improve the quality of flue-cured tobacco. (3) in the five of the organic fertilizer, the effection of oil and straw is good.