Abstract: Phytophthora root rot caused by Phytophthora sojae is one of main diseases in Heilongjiang soybean production. This disease have happened in the main soybean production areas of our country and led to a large quantity of outputs lose in some areas. Planting resistant cultivar is the most effective methods. So this research was focused on screening Phytophthora root rot resistant cultivars and resistance lines in Heilongjiang areas, and offered reference for controlling local disease and rational layout of disease-resistant cultivars. We used the hypocotyl vulnus inoculum to vaccinate 126 soybean cultivars and 135 portions of soybean lines, in order to identify eight main Phytophthora sojae strains. Our results showed that 72 portions of cultivars are resistant to more than 4 strains, occupy 57.1% of identified cultivars, and 84 portions of lines are resistant to more than 4 strains, occupy 62.2% of identified lines. Genes analysis of cultivars indicated that 30 soybean cultivars contained disease-resistant genes, 3 major genes, such as Rps1k, Rps3a and Rps1c, are included in 10 cultivars.