Abstract: To study acaricidal activity of compounds from Stellera chamaejasme L, three flavonoids were isolated from EtOAc and n-butanol extract of S. chamaejasme L by using various chromatographic techniques, and their chemical structure were identified as daphnelantoxin A (1), quercetin (2) and (+)-epiafzelechin (3) on basis of comparing spectroscopic data with literature. Their half lethal concentration (LC50) values against Tetranychus cinnabarinus B were detected as 4.9434 mg?mL-1, 7.6930 mg?mL-1, 2.2494 mg?mL-1 in acaricidal activity test, respectively. The result showed that epiafzelechin possess more better acaricidal activity.