Abstract: This test is to establish a new environmental safety evaluation technology for transgenic cotton in the national project of “12th Five-Year Plan”,which is aim at cultivating new variety of transgenic organism. The objects of this test are transgenic Cry1Ac plus Cry2Ab cotton and transgenic Cry1Ac plus EPSPS cotton, the CK are ganmian za No.1 and ganmian No.11, The former is the hybrid cotton, the latter is non-transgenic cotton.The test is carried out at garden of Verticillium Wilt by the methord which is different from disease-resistant identification,detect incidence of Verticillium dahliae, growth and productioncompetitiveness of the two transgenic bivalent gene cotton.The resistance to verticillium wilt of two objects is not significantly stronger than the two CK; the height and coverage competitiveness of transgenic Cry1Ac plus EPSPS cotton are relatively strong, that of transgenic Cry1Ac plus Cry2Ab cotton are relatively weak; the boll number and fruit branch layer of transgenicCry1Ac plus EPSPS cotton are more than CK,and that of transgenic Cry1Ac plus Cry2Ab cotton are less than CK;the drop rate of two objects is not significantly with two CK, seed cotton and lint yield of transgenic Cry1Ac plus EPSPS cotton are betweentne two CK,and that of transgenic Cry1Ac plus Cry2Ab cotton are lower than two CK. The results show that the planting feasibility of transgenic Cry1Ac plus Cry2Ab cotton is relatively poor, and transgenic Cry1Ac plus EPSPS cotton is general.