Transformation of Nicotiana alata with RNAi Vector of Trehalase Gene

doi:10.11924/j.issn.1000-6850.2014-0105

Abstract

Abstract: In order to make better use of flower tobacco in landscaping, its ability to resist abiotic stress need to be further enhanced. Trehalose plays an important role in plant resistance. Using RNA interference to inhibit the expression of trehalase gene can block the process of trehalose degradation, so as to increase the trehalose content in plant, which can be expected to improve plant resistance to abiotic stress. In this experiment, agrobacterium carried the RNA interference vector of Arabidopsis trehalase gene (iTre- 1285) had been transformed into flower tobacco (Nicotiana alata) by leaf disc infection method; positive plants were acquired after screening with kanamycin and carbenicillin. DNA was extracted from the positive plants and the target gene appeared in PCR detection. This meant that the target gene had been transformed into flower tobacco plants. So through this study, the genetic transformation methods of flower tobacco had established, while RNAi trehalase gene transformed into flower tobacco successfully. This result laid a foundation for cultivating transgenic flower tobacco against abiotic stress for further.

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