Optimization of Extraction Technology of Polysaccharide from Perilla Leaf Assisted by Microwave Technology

doi:10.11924/j.issn.1000-6850.2013-2392

Abstract

Abstract: The process conditions for perilla leaf polysaccharide were studied. Perilla leaf polysaccharide was extracted by microwave-assisted. The experimental factors, such as ration of solid to solvent (g/mL), microwave power, microwave time, and load were investigated. The result showed that load had remarkable influence on the extraction rate of polysaccharide; Microwave power and microwave time had significant effect on the extraction rate of polysaccharide. The optimum extraction conditions of perilla leaf polysaccharide by microwave were obtained as follow: load 10 mL, microwave time 30 s, microwave power 800 W. Under these conditions, the extraction rate of perilla leaf polysaccharide was about 3.99%. The extraction effect of perilla leaf polysaccharide was strengthened by microwave technology.

References

[1] 陆洁静,任文彬.紫苏的研究概况[J].农产品加工·学刊,2009(6):32- 34.
[2] 李海丽,史晓蓉.紫苏的应用现状及发展前景[J].农业科技与装备, 2011(07):66-67,71.
[3] 张卫明,石雪萍.紫苏全草营养成分测定[J].食品研究与开发,2009, 30(2):132-135.
[4] 魏国江,尤秋实,潘冬梅,等.紫苏营养成分及其在健康食材中的应用[J].黑龙江科学,2012,3(11):59-60.
[5] 谭美莲,严明芳,汪磊,等.国内外紫苏研究进展概述[J].中国油料作物学报,2012,34(2):225-23.
[6] 丁友芳,闫林林,王彩云,等.不同产地紫苏叶 HPLC指纹图谱研究[J].北京林业大学学报,2011,33(6):181-185.
[7] 陆风.紫苏和鸭跖草抗菌活性的研究[J].中国民族民间医药杂志, 2009,18(17):22-24.
[8] 邹盛勤,刘坤,陈武.紫苏不同部位中乌索酸和齐墩果酸的含量比较[J].食品工业科技,2008,29(04):284-286.
[9] 薛山.紫苏精油的研究新趋势[J].中国食品添加剂,2011(1):199- 204.
[10] 李鹏,朱建飞,唐春红.紫苏的研究动态[J].重庆工商大学学报:自然科学版,2010,27(3):271-275.
[11] 王玉萍,杨峻山.紫苏类中药化学和药理的研究概况[J].中国药学杂志,2003,38(4):250-253.
[12] Peng Y Y, Ye J N, Kong J L. Determination of phenolic compounds in perilla frutescens L. by capillary electrophoresis with electrochemical detection [J]. J. Agric. Food Chem,2005(53):8141- 8147.
[13] 刘月秀,张卫明.紫苏属植物的研究与利用[J].中国野生植物资源, 1996(3):24-27.
[14] 马尧,庄云.紫苏不同部位总黄酮含量测定[J].安徽农业科学,2010, 38(33):18775-18776.
[15] 张鑫,张志军,李会珍,等.紫苏叶花青素的提取及测定[J].食品研究与开发,2009,30(9):187-190.
[16] 郭群群,杜桂彩,李荣贵.紫苏叶挥发油抗菌活性研究[J].食品工业科技,2003,24(9):25-27.
[17] 张旭,吴卫,郑有良,等.紫苏属植物中不同部位无机元素及维生素分析[J].时珍国医国药,2008,19(6):1383-1385.
[18] Sanbongi C, Takano H, Osakabe N, et al. Rosmarinic acid in perilla extract inhibits allergic inflammation induced by mite allergen, in a mouse model [J]. Clinical & experimental allergy, 2004, 34(6):971- 977.
[19] 王钦富,王永奇,于超,等.炒紫苏子醇提取物对过敏模型小鼠的抗过敏作用及机制[J].中草药,2006,37(10):1532-1535.
[20] 刘娟,雷焱霖,唐友红,等.紫苏的化学成分与生物活性研究进展[J].时珍国医国药,2010,21(7):1768-1769.
[21] 朱建明,魏艳.紫苏油的研究现状及应用前景[J].中国中医药信息杂志,2001,8(10):33-34.
[22] 黄丹,刘达玉.紫苏提取物抑菌特性研究[J].食品工业,2007,28(3): 11-13.
[23] Kawahata T, Otake T, Mori H, et al. A novel substance purified from Perilla frutescens Britton inhibits an early stage of HIV- 1 replication without blocking viral adsorption [J]. Antivir Chem. Chemother. 2002,13(5):283-288.
[24] Qiao S, Li W, Tsubouchi R, et al. Rosmarinic acid inhibits the formation of reactive oxygen and nitrogen species in RAW264.7 macrophages [J]. Free radical research, 2005,39(9):995-1003.
[25] 天瑶,金安娜,陈轶玉,等.紫苏油对衰老小鼠脑组织 GSH含量的影响[J].中国油脂,2004,29(2):68-70.
[26] 夏志颖.紫苏栽培管理技术[J].天津农林科技,2012(4):22-23.
[27] 田海娟.超高压灭菌技术在紫苏豆酱中的应用[J].中国调味品, 2013,38(3):80-83.
[28] 秦晓霜,康笑枫,林春华,等.紫苏挥发油化学成分分析[J].广东农业科学,2006(6):36-37.
[29] 李燕,曾庆华,陈利梅.正交试验法优选紫苏叶中总黄酮提取工艺[J].农业机械,2011(12):148-150.
[30] 丁保金,金丽琴,吕建新.多糖的生物活性研究进展[J].中国药学杂志,2004,39(8):561-564.
[31] 吕长鑫,李萌萌,徐晓明,等.响应面分析法优化纤维素酶提取紫苏多糖工艺[J].食品科学,2013,34(2):6-10.
[32] Kwon K H, Kim K I, Jun W J, et al. In vitro and in vivo effects of macrophage- stimulatory polysaccharide from leaves of perilla frutescens var. crispa [J]. Biological & pharmaceutical bulletin, 2002, 25(3):121-130.
[33] 易立涛,王星.半夏厚朴汤君药配伍抗抑郁效应研究[J].中国天然药物,2010(5):56-60.