Abstract: The less rainfall in spring in Loess Plateau of Inner Mongolia affects the emergence and yield of millet seriously, so it is a matter of great urgency to improve drought resistance of seedling of crops. This paper examined the seedling, soil moisture and yield of millet applied with PAA and different water rates. PAA application combined with water in the 6 treatments were 0 L/hole (P₀), 0.1 L/hole (P₁), 0.2 L/hole (P₂), 0.3 L/ hole (P₃), 0.4 L/hole (P₄) and control (CK), and emergence rates, soil moisture and yield of millet were measured. The emergence of PAA combined with water treatments were 3-6 days earlier than that of CK and P0, the seedling emergence rate were P₃＞P₄＞P₁＞P₂＞CK＞P₀. Treatments applied with PAA improved the emergence uniformity, plant height, stem diameter, root length, fresh and dry weight of millet and soil moisture. The soil volumetric water contents in the layers of 5 cm, 10 cm, 15 cm, 20 cm, 30 cm increased by 4.6%-11%, 1.2%- 15.5% , 1.3%- 5.7% , 0.9%- 3.8% and 1.2%- 4.6% , respectively. In the years of 2012 and 2013, compared with the control (CK), the yields under the application of a PAA combined with irrigation increased by 9.50%-32.43% and 14.88%-35.47%, respectively, with the increasing order as P₃＞P₄＞P₁＞P₂. Therefore, applying PAA combined with irrigation in millet could improve the germination rate and uniformity, promote the seedling growth and increase yield, and the optimization was applying PAA with irrigation 0.3 L/hole and 0.4 L/hole.