Abstract: The aim of the study was to effectively suppress harm to peanuts in the condition of peanut cropping autotoxins. By screening the strains, which could degrade peanut autotoxicity benzoic, we investigated the degradation and antagonistic effect. Using the benzoic acid as sole carbon source, the methods of benzoic acid concentration were increased gradually. three degrading strains were screened, the degradation rate of the benzoic acid were 95.32%, 91.63% and 90.15%, the three strains on peanut root secretion from the autotoxicity had a strong degradation effect, while three strains of Fusarium oxysporumthere is a strong antagonistic. The results of pot experiment showed that by the applying of the three strains could effectively alleviate the inhibitory effect of autotoxicities of peanut seedlings, increase yield significantly. The three strains had the double function of detoxification and antibacterial. They could be effectively used for microfertilizer in the agricultural production of crops in the future and improve the soil environment, providing a theoretical basis.