Abstract: In order to effectively eliminate the severe disturbances of the secondary substances, polysaccharides and polyphenolics and obtain a high-quality RNA from the tissues of Angelica sinensis for quantitative RT-PCR, three methods of CTAB, Kits and Trizol reagent were used for isolations of total RNA from the roots, stems and leaves of Angelica sinensis, respectively. The purity and yield of the total RNA isolated were detected by a UV/VIS spectrometer, the integrity of total RNA isolated was analyzed by the nondenaturing agarose gel electrophoresis and the quality of total RNA isolated was validated by the quantitative RT-PCR. The results showed that RNAs of the roots, stems and leaves isolated by CTAB method had a better integrity and purity, and the yield of leaves was higher than that of the roots and stems. The total RNAs isolated by Kits method had a good integrity, but there were a lower yield and poorer purity; RNAs of the stems by Trizol reagent could be used for the quantitative RT-PCR, but RNAs of the roots and the leaves were highly polluted, which was ineligible for the subsequent operations. Therefore, CTAB method could be indicated as an economical, reliable and applicable method for isolating total RNA from Angelica sinensis for quantitative RT-PCR. This work established a methodology basis for the research on the molecular biology of Angelica sinensis, and also provided a reference for the total RNA extraction of other medicinal plants.