Abstract: In order to understand the changes in the concentration of CO2 greenhouse characteristic in alpine cold regions, forecast greenhouse CO2 concentration, CO2 concentration provide the basis for regulating greenhouse. According to April 2012 to March 2013 the state of modern agricultural demonstration park in Datong County of greenhouse gas concentrations of CO2, inside and outside the greenhouse meteorological observations, and Datong meteorological observation data, the author analyzed alpine cold regions under different weather types greenhouse CO2 concentration variation. The results showed that: the CO2 concentration in the range of 184-577 mg/kg during the test, daily average was 383 mg/kg, minimum and daily average showed a significant upward trend; regardless of the weather types, CO2 concentration diurnal variation showed ‘U’ shaped curve, strong growth of crops, natural ventilation could not be avoided CO2 deficit; CO2 concentration in the cloudy sky and the minimum surface temperature at the best correlation between the maximum, sunny and cloudy days with daily average temperature correlation between the best. In addition to March 2013, each month had shown a deficit phenomenon of CO2, CO2 deficit duration and daily had a relationship between the minimum CO2 concentrations, which was a quadratic curve.