Abstract: The experiment use‘Yanshu4’,‘Dongnong311’,‘Kexin13’,‘Kexin18’as the tested materials, in order to research the absorption and accumulation of Fe in tubers of different potato varieties during sampling period,to provide a reasonable basis for potato quality improvement. The results showed: during all the sampling stages, the concentrations of iron in tubers of different potato varieties show a similar patter, reducerise-reduce-rise patter, but in different varieties, the rise and reduce times were different. The maximum value appeared in the first sampling period; the tuber iron concentration of 4 varieties was‘Dongnong311’＞‘Kexin13’＞‘Yanshu4’＞‘Kexin18’, the range was 41.8-51.2 mg/kg. Iron accumulation in tubers of four varieties showed a trend of rising over all the growth period basically, and the correlation of iron accumulation and dry matter accumulation in tubers of four varieties was significant.