Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2014, Vol. 30 ›› Issue (27): 265-269.doi: 10.11924/j.issn.1000-6850.2014-0769

Special Issue: 生物技术

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Cloning and Expression of A Chitinase Gene chiB

  

  • Received:2014-03-20 Revised:2014-03-20 Accepted:2014-05-27 Online:2014-10-15 Published:2014-10-15

Abstract: Chitinase can play the role of insect resistance by destroying chitin in insect tissues. In order to get the plant expression plasmid of chitinase, homology cloning method was used to separate chiB gene from Srratia marcescens, the length was 992 bp; pET28 expression vector was constructed and the gene expression was induced by IPTG. The analysis showed that the gene expression product belonged to glycosyl hydrolases family 18, and plasmid pCAM-35S-chiB was constructed by pCAMBIA2300.