Colonization of NJQG-3A1 Strain of Endophytic actinomycetes and Its Control Effect on Banana Fusarium Wilt

doi:10.11924/j.issn.1000-6850.2014-1001

Abstract

Abstract: The colonization capacity of NJQG- 3A1 strain in banana plants and the rhizosphere was investigated, and the control efficacy of NJQG-3A1 against banana fusarium wilt was also studied, which will provide a theoretical basis for ecological control of the disease. The colonization trends of NJQG- 3A1 in banana plants and the rhizosphere was examined with the method of antibiotic-resistance. Furthermore, the effect of NJQG-3A1 against banana fusarium wilt was investigated by using pot experiment method. Results showed that NJQG- 3A1, tagged by the resistance of‘Ampicllin (100μg/mL)- Potassium dichromate (100μg/mL)’, could colonized in roots, corms and pesudostems tissue of banana and rhizosphere soil stably. The population of NJQG-3A1 in different part was: rhizosphere soil＞roots＞corms＞pesudostems＞leaves. It was also found that the population colonied in the rhizosphere and the banana plant was 1×10⁵ cfμ/g, 1×10² to 1×10³ cfμ/g respectively. In addition, the pot experiment result showed that the banana plant height, steam circumference, fresh weight were increased by 99.22%, 44.60%, 218.67% respectively, significantly different from the control (P＜0.05), when treated with the strain NJQG-3A1 fermentation liquid strain NJQG-3A1 also showed obvious biocontrol effect on banana banana fusarium wilt, the disease indexes of leaves and root-steam were 15, 10 respectively, and the control efficacy were 81.82% , 88.98% , respectively. NJQG- 3A1 could colonize in the banana plant and the rhizosphere. The strain NJQG- 3A1 fermentation liquid showed strong disease biocontrol effect on banana fusarium wilt, as well as promotion effect on banana plant growth.

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