Abstract: Using the analytical methods of Mann-Kendall, linear trend evaluation and correlation analysis, the temporal- spatial distribution law of winter accumulate temperature, the fundamental features and changing trend of winter wheat growth stages and the impact of winter accumulate temperature on winter wheat growth stages were identified in this study based on historical data of winter wheat growth stages of 9 agrometeorological observatories and meteorology data of 35 meteorological stations from 1960 to 2010. Results indicated that: (1) the winter accumulate temperature increased significantly since the early 1990s in north area of Huaihe river and the middle 1990s in south; (2) the growth period lengths (GPL) from sowing to jointing and that after jointing were shorter in south than in north, the regional disparity of the GPL from sowing to jointing was the largest, and the regional disparity of the GPL from sowing to booting and that after booting decreased gradually, when the regional disparity was smaller in south than in north; (3) the GPL from sowing to jointing shortened significantly in the past 30 years, while the GPL from jointing to ripening prolonged significantly, and the GPL from flowering to milk-ripening which was very important to the output was also prolonged obviously; (4) with the winter accumulated temperature increasing, the GPL from sowing to overwintering, jointing to ripening and flowering to milk-ripening prolonged significantly, while the GPL from overwintering to jointing, sowing to jointing shortened significantly. Winter wheat is one of the main food crops, analysis of the impact of winter accumulate temperature on its growth stages could supply basis for knowing well the changing rule of each growth stage and provide necessary agricultural guidance.