Abstract: To screen favorable flowering characteristics under high temperature and summer drought, twentythree Japonica male sterile lines screened with high temperature and summer drought and CK withⅡ-32A were systematically observed. Results indicated that every tested variety had significant difference on flowering characteristics. Compared with CK, 35478A, 35489A and 35502A had the advantages that the flowering peak were comparatively earlier, the flowering duration of single plant and single panicle were longer, the rate of grain in neck were lower, the opening angle of spikelet were bigger and the distance of opened glume tips were longer. The flowering peak of 35478A was during 10:00-11:00 and 1.5 h earlier than that of CK. The stigma exertion rate of 35489A was 76.23% and higher than that of CK. 35502A had higher stigma vitality, the highest was 84.68%, and the lasting time kept longer than CK. 35478A, 35489A and 35502A had good flowering characteristics.