Abstract: A very important way to increase cassava production is to optimize the amount and proportion of fertilizer. The field experiment was designed using new cassava varieties‘SC7’to explore the effect of fertilization on soil nutrition, soil enzyme activity and plant growth. Results showed that fertilization could promote soil acidification and increase soil pH. The more fertilization applied, the more severe acidification could occur. It could reach the highest value of acidification and soil organic material when organic and compound fertilizer was mixed. Soil nitrogen, phosphorus content and potassium in D fertilizer treatment was the highest, in particular, phosphorus content and potassium was apparently higher than A fertilizer treatment (CK). Fertilization can increased soil urease, phosphatase activity and catalase activity overall, in particular, D fertilizer treatment played more significant role in improving the soil enzyme activity. More organic fertilizer played more effective role in improving soil fertilizer and increasing soil enzyme activities. E fertilizer promoted the plant growth more effective than the application of single organic fertilizer. Then, selection of organic and compound fertilizer can both keep the soil fertility and promote the growth of cassava to achieve high yield.