Abstract: The status and content of physiological components in the stigmas and styles were important to the germination and growth of pollen tubes. Soluble sugar, protein, acids, phenolic contents and POD isozyme activity in stigmas and styles of different Nicotiana species was determined by phenol method, coomassie brilliant blue, ninhydrin solution staining method, the forint method and guaiacol method. The results showed that the content and activity of different physiological components changed irregularly by the species and development stages. Acids content in stigmas was lower than that in styles among all the Nicotiana species and the same was true of soluble sugar apart from N. Stocktonii. On the contrary, phenolic contents in stigams was higher than that in styles and the same was true of POD isozyme activity apart from N. repanda. Protein contents in stigmas of [(gla.s) K326] and K326 was higher than that in styles.