Abstract: Alcohol dehydrogenase (ADH) is widely distributed and plays an important role in various tissues of plant. The cloning and expression analysis of banana ethanol dehydrogenase gene under adversity stress were laid the foundation for further research the ADH gene function in banana. The cloning of a ADH cDNA, designated as MaADH gene, was isolated from banana fruit by Suppression Subtractive Hybridization (SSH) and gained by RACE technique. The cDNA was 1140bp in length, coding for 379 amino acid residues. Predicted by bioinformatics analysis, its protein molecular weight is 40.7 KDa, isoelectric point 7.09. By conserved domain analysis, the gene has 22 NAD binding sites, 11 substrate binding sites and 4 catalytic Zn binding sites. Analyzed by phylogenetic tree, MaADH gene is more closely related to Arabidopsis and Brassica oleracea. MaADH gene stress expression analysis showed that the MaADH expression of banana seeding dealt with salt and cold upregulated, and drought firstly upregulated, and then down-regulated, while dealt with wounding upregulated, and then down-regulated, but did not change significantly. Research shows that the banana alcohol dehydrogenase gene plays an important role in banana which adapted to salt, low temperature, drought and damage and so on.